底物-Lumigen APS-5 Lumigen ®的APS - 5采用了独特的技术，在化学发光检测过程中，为碱性磷酸酶偶联提供优越的灵敏度和易用性。反应由acridan(9,10-二氢吖啶)作为底物和碱性磷酸酶标记，生产持续高强度的化学发光。Lumigen ®APS - 5是溶液分析法测定磷酸酶活性和磷酸酶酶联免疫检测非常理想的解决方案。可以用化学发光酶标仪的微孔板中进行的碱性磷酸酶检测，来体现Lumigen ®APS – 5的灵敏度 ELISA AP Substrate Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of Lumigen APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.  Excellent sensitivity - low picogram to femtogram Rapid peak intensity - reduced assay time and increased throughput Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves Rapid Peak Intensity The light-output peak intensity of Lumigen APS-5 reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm. Lumigen APS-5 is available in 100 mL and 1 Liter volumes. Visit the Lumigen store to purchase on-line. Detection Technology Chemiluminescence Download Data Sheet  Application Instructions  Handling Instructions  MSDS  Product Specifications Enzyme  Alkaline Phosphatase (AP) Application  ELISA Sensitivity  Low picogram to femtogram Storage Conditions  2-8° C; Store in amber bottle to protect from light Stability  1 year stored at 2-8° C Working Solution  Ready to use solution