ELISA AP Substrate

Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of Lumigen APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus. 

• Excellent sensitivity - low picogram to femtogram
• Rapid peak intensity - reduced assay time and increased throughput
• Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control
• Sustained luminescence - timing is less critical with light intensities able to be read at any time to produce linear calibration curves

Rapid Peak Intensity

The light-output peak intensity of Lumigen APS-5 reaches sustained maximum within seconds of substrate addition. Light emission is maximal at 450 nm.